補益中藥對運動性腎缺血再灌注損傷大鼠腎臟炎癥因子及 ECM 表達的影響
鄭孟君1 ，曹建民2 ，郭 嫻2 ，周海濤3
(1． 江南大學 體育學院，江蘇 無錫 214000;2． 北京體育大學 運動人體科學學院，北京 100084;
3． 北京聯合大學 生物化學工程學院，北京 100023)
摘 要: 觀察補益中藥對運動性腎缺血再灌注損傷大鼠腎臟炎癥因子及 ECM 表達的影響。方法:75 只 7 周齡雄性Wistar 大鼠隨機分為 4 組:安靜對照組( C 組，12 只) 、一般訓練組( M 組，12 只) 、過度訓練組( OM 組，24 只) 和補益中藥 + 過度訓練組( TM 組，24 只) ，M、OM、TM 組進行 8 周 56 天的游泳訓練。采用專業灌胃器每天灌胃 1 次，TM 組灌胃采用黃芪、紅景天、丹參、苦參等組成的復合中藥制劑，劑量為 1 g·kg － 1 ，體積為 5 mL·kg － 1 ，其他各組灌胃等量生理鹽水。末次訓練后 24 h，采用 PAS 染色觀察腎小球 ECM 沉積情況，免疫組織化學法檢測腎組織 TNF-α、IL- 1β、IL-6、IL-18 蛋白表達，RT-PCR 法檢測腎組織 TNF-α mRNA、IL-1β mRNA、IL-6 mRNA、IL-18 mRNA、TGF-β1 mRNA表達。結果:1)8 周實驗后，腎小球 ECM 沉積，C、M 組間無顯著差異( P ＞ 0. 05 );OM 組較 C、M 組顯著增加( P ＜
0. 01);TM 組顯著低于 OM 組( P ＜ 0. 05)。2) 血尿素氮和血清肌酐水平，OM 組和 TM 組顯著高于 C 組( P ＜ 0. 01)， TM 組顯著低于 OM 組( P ＜ 0. 05);腎組織 TNF-α、IL-1β、IL-6 和 IL-18 蛋白表達，OM 組和 TM 組顯著高于 C 組( P ＜
0. 01) ，TM 組顯著低于 OM 組( P ＜ 0. 05);腎組織 TNF-α mRNA、IL-1β mRNA、IL-6 mRNA 和 IL-18 mRNA 表達，OM、 TM 組顯著高于 C 組( P ＜ 0. 01) ，TM 組顯著低于 OM 組( P ＜ 0. 05);腎組織 TGF-β1mRNA 表達，TM 組( P ＜ 0. 05) 和 OM 組( P ＜ 0. 01) 顯著高于C 組，TM 組顯著低于OM 組( P ＜ 0. 05)。結論:8 周過度訓練致大鼠發生運動性腎缺血再灌注，同時 ECM 沉積加強。補充補益中藥可通過抑制腎臟組織 TNF-α、IL-1β、IL-6、IL-18 的表達進而減輕了過度訓練誘導腎臟缺血再灌注發生時腎臟組織 TGF-β1 的表達，抑制 ECM 的合成和( 或) 促進 ECM 的降解及組織病理學改變，延緩或避免過度訓練導致的運動性缺血再灌注對腎臟的損害。
關鍵詞: 補益中藥;運動性腎缺血再灌注損傷;炎癥因子;細胞外基質
中圖分類號: G804． 7 文獻標志碼: A 文章編號: 1004 － 0560( 2015) 03 － 0068 － 06
Effect of Tonic Medicine on Kidney Inflammation Factors and ECM Expression in Rats of Exercise-related Renal Ischemia-reperfusion Injuries
ZHENG Mengjun1 ，CAO Jianmin2 ，GUO Xian2 ，ZHOU Haitao3
(1． Sports College，Jiangnan University ，Wuxi 214000，Jiangsu，China;
2. Sport Science College of Beijing Sport University ，Beijing 100084，China;
3. Biochemical Engineering College of Beijing Union University ，Beijing 100023，China)
Abstract:Objective:To study the protective effects of tonic medicine on kidney inflammation factors and extracellular ma- trix ( ECM ) expression in rats of exercise-related renal ischemia-reperfusion injuries． Methods:75 male Wistar rats at the age of 7 weeks were divided into 4 groups randomly:control group( C group，n = 12) ，general training group( M group，n =
12) ，overtraining group( OM group，n = 24) ，tonic medicine + overtraining group( TM group，n = 24) ，the Rats in M ， OM ，TM groups were assigned to 8 weeks of swimming training，7 days a week． Professional gavage were given once a day． The Rats of TM group were given with compound Chinese herbal medicine( The primary elements were Chinese drugs for invigoration，such as Astragalus，Rhodiola rosea，Salviae miltiorrhizae，Sophora flavescens) ，gavages( ig) dose was 1 g
· kg － 1 ;ig volume was 5 mL·kg － 1 ，and other groups were given the normal saline． 24 hours after the last training，w e ob-
served the glomerular ECM deposition by the PAS stain;detected the renal tissue of TNF-α，IL-1β，IL-6，IL-18 protein by immunohistochemistry and detected the expression of TNF-α mRNA，IL-1β mRNA，IL-6 mRNA，IL-18 mRNA，TGF-β1 mRNA gene in renal tissue by RT-PCR method． Results:1)8 weeks after the experiment，the glomerular ECM deposition of C group and M group appeared normal and showed no differences( P ＞ 0． 05);OM group was significantly higher than that in C group and M group( P ＜ 0． 01) ，TM group was significantly lower than that of group OM ( P ＜ 0． 05)． 2) OM
收稿日期: 2015-03-04; 修回日期: 2015-03-29
基金項目: 國家體育總局課題(2013A101)。
作者簡介: 鄭孟君(1980—) ，女，講師，碩士，主要研究方向為體育教學與運動訓練。通信作者: 周海濤(1976—) ，男，副教授，碩士，主要研究方向為運動性疲勞與恢復。
第 3 期 鄭孟君，等: 補益中藥對運動性腎缺血再灌注損傷大鼠腎臟炎癥因子及 ECM 表達的影響 ·69·
group and TM group blood urea nitrogen and serum creatinine were significantly higher than that of C group( P ＜ 0． 01)， TM group was significantly lower than that of group OM ( P ＜ 0． 05);the expression of renal TNF-α，IL-1 β，IL-6 and IL- 18 protein in OM group and TM group was significantly higher than that in C group( P ＜ 0． 01) ，TM group was significant- ly lower than that of group OM ( P ＜ 0． 05);the expression of renal TNF- α mRNA，IL-1 β mRNA，IL-6 and IL-18 mRNA in TM group and OM group was significantly higher than that of C group( P ＜ 0． 01) ，TM group was lower than of OM group( P ＜ 0． 05);the expression of renal TGF-β1mRNA in TM group and OM group was significantly higher than that of C group( TM ，P ＜ 0． 05;OM ，P ＜ 0． 01) ，TM group was significantly lower than of OM group( P ＜ 0． 05)． Conclusion: 8 weeks overtraining caused the movement of rat renal ischemia reperfusion and increased the deposition of ECM ． Tonic medicine can restrain the expression of renal tissue TGF-β1 during exercise-related renal ischemia-reperfusion injury in- duced by overtraining，w hich may be achieved by inhibiting the expression of IL-1，TNF-α，IL-6，IL-18 in renal tissue． Fi- nally it decreased the synthetic and increased the decomposition of ECM ，reduced the damage and the histopathological changes，as a result，Tonic medicine can protect the rats from exercise-related renal ischemia-reperfusion injury．
Key words:tonic medicine;exercise-related renal ischemia-reperfusion injury;inflammation factors;extracellular matrix
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